Insertion junction: CLIP2.060573_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060573
Chromosome:chromosome 11
Location:4376689
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482900 RAB20,RAB18C,RABC3 Small Rab-related GTPase; (1 of 3) K07910 - Ras-related protein Rab-18 (RAB18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGATAACGCCAAGCCATCAAGTAGGGGCGCAAGGGAAGGCGGGTTGCT
Internal bar code:ACCGTGGATATCTAGAAACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2385
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCATCAATCAGCAGCTGC
Suggested primer 2:GAAGCATAGCGTTCAAGCGG