| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060574 |
| Chromosome: | chromosome 12 |
| Location: | 7424831 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557252 | (1 of 1) K18985 - engulfment and cell motility protein 2 (ELMO2, CED12A) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGGAATCGTCGCTTGCAGAGCGCGGTTCTACATCGGCGCAGCGTGT |
| Internal bar code: | TGTTCATGGAAGATATTATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5172 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAATCCATGAGCGGCAAG |
| Suggested primer 2: | CAAATGGCCCGCTATTCTGC |