Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.060588 |
Chromosome: | chromosome 7 |
Location: | 2995026 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g333400 | MMP23 | Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin | 3'UTR |
Cre07.g333450 | SGT1 | Sterol 3-beta-glucosyltransferase; (1 of 2) 2.4.1.173 - Sterol 3-beta-glucosyltransferase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAAGCGTAAGTGGGTTCACAACTTACAGCACTGGCGTGCCACAACCC |
Internal bar code: | GCTCGAGCCATCGAGTCTGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1919 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATGGCCGTGTGAAGTAGCA |
Suggested primer 2: | GACGAGGGGAAAGCTGTTGA |