| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.060605 |
| Chromosome: | chromosome 10 |
| Location: | 3034710 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440800 | (1 of 88) IPR011333 - POZ domain | outside_mRNA | |
| Cre10.g440850 | GPX4 | Glutathione peroxidase 4; (1 of 2) 1.11.1.12 - Phospholipid-hydroperoxide glutathione peroxidase / Peroxidation-inhibiting protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAACGAATGCAGGGCCTGCTGCTGCGCCAACTCTTGCGCGGAAGCAG |
| Internal bar code: | TTTGTAATCTTTAACTTCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1414 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAGGTATCCCCGTTACG |
| Suggested primer 2: | GCCTGGCACATGTCTAGGTT |