Insertion junction: CLIP2.060615_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060615
Chromosome:chromosome 7
Location:6046485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g355050 (1 of 1) IPR003610 - Carbohydrate-binding module family 5/12 3'UTR
Cre07.g355100 CrZIP5,ZIP1 (1 of 6) K14709 - solute carrier family 39 (zinc transporter), member 1/2/3 (SLC39A1_2_3, ZIP1_2_3); Zinc/iron transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTACACCAGATTGCCGCCCAGGTGTCCAAGTCGCGGGCAGAGCACATGC
Internal bar code:GACGGGGCAGCACTCCAGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3652
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACGTGTGCGATATCGT
Suggested primer 2:TACTACAACACTGGCGAGCG