Insertion junction: CLIP2.060622_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060622
Chromosome:chromosome 17
Location:4523359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g731466 (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain gene_edge/mRNA_edge/3'UTR
Cre17.g731500 FAP394 Flagellar Associated Protein 394; (1 of 1) IPR000157//IPR011050 - Toll/interleukin-1 receptor homology (TIR) domain // Pectin lyase fold/virulence factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATTACATCTCAACAGCTCGCCTCCGATCCTCTGTCACTACGTGCGACC
Internal bar code:TTACGGCATATGTTGTATTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:638
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTCACGCAACCCCTAAT
Suggested primer 2:CTTCAACACCAACGTGCAGG