| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060622 |
| Chromosome: | chromosome 17 |
| Location: | 4523492 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731466 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | gene_edge/mRNA_edge/3'UTR | |
| Cre17.g731500 | FAP394 | Flagellar Associated Protein 394; (1 of 1) IPR000157//IPR011050 - Toll/interleukin-1 receptor homology (TIR) domain // Pectin lyase fold/virulence factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCGCTGGGCCAGTATTAACAACATTCCCCCCTCCTTTCCCACTTCC |
| Internal bar code: | TTACGGCATATGTTGTATTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1385 |
| LEAP-Seq percent confirming: | 56.5217 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAACACCAACGTGCAGG |
| Suggested primer 2: | CCACTCACGCAACCCCTAAT |