Insertion junction: CLIP2.060644_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.060644
Chromosome:chromosome 13
Location:1920942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576050 ZIP2,CrZIP10 (1 of 1) PTHR11040:SF61 - PROTEIN ZNTA; Zinc/iron transporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGGGCAACGGTGTCTTTCTGGCGCTGGTGGTTAGATGCATCAGCCGAC
Internal bar code:AACAACAATTGATAGACTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3185
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAAGTGTTTGCACCTGC
Suggested primer 2:GGCACTACTTCCACCGTTCA