| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060652 |
| Chromosome: | chromosome 8 |
| Location: | 4449450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385650 | (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGGCGAGCGTGGACCGCCCTGCCACTCAACCAGTAAGTCAATGCTAT |
| Internal bar code: | GAGTTTTGATATGGGCAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4262 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCAGTCAGTAGCGGGTG |
| Suggested primer 2: | GCTGCTTGTGTGTTGACTGG |