Insertion junction: CLIP2.060659_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.060659
Chromosome:chromosome 17
Location:2751093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717800 NUP93 Nucleoporin 93; (1 of 1) K14309 - nuclear pore complex protein Nup93 (NUP93, NIC96) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAGGACGCAAGGACCCGCGGCACAAAGCGTGTGCGTGGCAGGCATGAC
Internal bar code:TTCCACGACACCAGAGTATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2292
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGTTTCACGGTCAGCCT
Suggested primer 2:AACAACCGTCCATCCATCCC