| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060670 |
| Chromosome: | chromosome 1 |
| Location: | 5515234 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039250 | RPS2 | Cytosolic 80S ribosomal protein S2; (1 of 1) K02981 - small subunit ribosomal protein S2e (RP-S2e, RPS2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGCACGGATTTTCCCTCTTTCGCTACTTAGTCGTAGCTCAACACCAA |
| Internal bar code: | ATTTATCCTCCGTTTACTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATATTTGCGTGCGCCATG |
| Suggested primer 2: | CTTACCGTATACCGCGTGCT |