Insertion junction: CLIP2.060675_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060675
Chromosome:chromosome 12
Location:9781127
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549852 (1 of 14) IPR002198//IPR002347 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTTTGGGTGCCGCGCATCTACCAGGTGTAGACGTTTTTGGCCGGTT
Internal bar code:ACCACGCGAGGCTACAAAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1980
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCAACCCTCATCACCCAC
Suggested primer 2:GCGCTGACTGTGATACCGTA