| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060677 |
| Chromosome: | chromosome 10 |
| Location: | 2925399 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439900 | HSP70G | ER-located HSP110/SSE-like protein; (1 of 1) K09486 - hypoxia up-regulated 1 (HYOU1) | 3'UTR |
| Cre10.g439950 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCTGGGCACGTGTGATGTACACGCGTCATGTTGACCAGCGTGCGAC |
| Internal bar code: | CTAACAAAAAAGTTACGTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3958 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCAGAGTCCAAAGCATCT |
| Suggested primer 2: | AGGAGGCGAAGAAGGAGGAT |