Insertion junction: CLIP2.060683_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060683
Chromosome:chromosome 5
Location:3182660
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g232050 (1 of 37) IPR008979 - Galactose-binding domain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAAATCCCCTCGACCCAGACCACCCCGCGCTCAACTCTCTGCCGCGTG
Internal bar code:CAAACAATATCGGCTTTAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2111
LEAP-Seq percent confirming:36.3636
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCACCGATGATTGCGCTG
Suggested primer 2:CTTGATGTCGTCATGCAGCG