| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060686 |
| Chromosome: | chromosome 11 |
| Location: | 3174859 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475900 | (1 of 2) PTHR12121//PTHR12121:SF34 - CARBON CATABOLITE REPRESSOR PROTEIN 4 // LD39302P | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTAGGACTCAGAGCAGAGGAGGCTGCGGGAACGCGGCCAGCAGCAGT |
| Internal bar code: | GTGGGCAAATTTTGGGAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3800 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCACGTCTGCTGCTAT |
| Suggested primer 2: | ACTTCCATATCCGGGTCCCA |