| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060688 |
| Chromosome: | chromosome 3 |
| Location: | 3911515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170850 | MMP19 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACCGGGGACGTGGTCCAGTCGCTCACACTCCATTCCGCCCACCCTC |
| Internal bar code: | TATGATAGTTCCTGACTAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1541 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTGGAGGAGAAGGAGA |
| Suggested primer 2: | CGACCTCTCTGTACCGCATC |