| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060690 |
| Chromosome: | chromosome 7 |
| Location: | 3535532 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336350 | (1 of 1) PTHR24012//PTHR24012:SF432 - FAMILY NOT NAMED // HEPHAESTUS | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTTGAGTGGTGGCGGAGGAGGGCCCCATGGACCCTTGCATGTAACGC |
| Internal bar code: | GGGTGCGCATGAATTCCGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1885 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGCGGGCTAGACATATG |
| Suggested primer 2: | TGGTAGGGATGACCAGGAGG |