| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060695 |
| Chromosome: | chromosome 1 |
| Location: | 4945188 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034300 | (1 of 1) PF09073 - BUD22 (BUD22) | 3'UTR_intron | |
| Cre01.g034350 | (1 of 2) PTHR10641//PTHR10641:SF585 - MYB-LIKE DNA-BINDING PROTEIN MYB // MYB DNA-BINDING-LIKE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCCCTGCTGCCGTGCACCACCTCCCAGGCCTTCGCCCTCAAACAGT |
| Internal bar code: | TTCGTTTTGGCCACGTAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2420 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGCGTAGTTCGTGTGT |
| Suggested primer 2: | GACCAACCGCCATTTTGCTT |