| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060703 |
| Chromosome: | chromosome 12 |
| Location: | 4190460 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517900 | SCA1,SECA1 | (1 of 1) K03070 - preprotein translocase subunit SecA (secA); Chloroplast-associated SecA protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGTCGTCGTTATACAGTTGCTTGATTGGCCTTTGCGTTTGCCATCA |
| Internal bar code: | GAGCAGATGAACTCAGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1554 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGTACGC |
| Suggested primer 2: | GTTGGGGAGCATTTGGAGGA |