Insertion junction: CLIP2.060717_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060717
Chromosome:chromosome 3
Location:4976808
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179400 MFT17 Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCGATGCACTAAAATTATTTTCTTAACCAAACATTTGTAAGCGTCTG
Internal bar code:GTCCAGTACGGTAGAAGTGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2107
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTGACACTGAAGCAGTCT
Suggested primer 2:CACTGTCAACTGCAAGACGC