Insertion junction: CLIP2.060720_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060720
Chromosome:chromosome 16
Location:3791010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688450 CFAP298,DAB2,C21ORF59,FBB18 (1 of 1) PF11069 - Protein of unknown function (DUF2870) (DUF2870); Flagellar basal body proteome 18 / Dynein assembly factor gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTACACAGCGTGTATCCGGAGAATTAGCGATTGGCTGCATGAGGTCA
Internal bar code:TGTTGGACTGTTTGATCGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2269
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGGTCCAGGTTGTAGG
Suggested primer 2:GAGGCCACAGTCGTCTCAAA