Insertion junction: CLIP2.060720_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060720
Chromosome:chromosome 16
Location:3791021
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688450 CFAP298,DAB2,C21ORF59,FBB18 (1 of 1) PF11069 - Protein of unknown function (DUF2870) (DUF2870); Flagellar basal body proteome 18 / Dynein assembly factor gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGTAATGTGCGTTATGCACTCCAAAATCGTGTAGCTAGTAAGCACTA
Internal bar code:TGTTGGACTGTTTGATCGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2531
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCCACAGTCGTCTCAAA
Suggested primer 2:CAGCAGGTCCAGGTTGTAGG