| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060725 |
| Chromosome: | chromosome 16 |
| Location: | 6995917 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g802000 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCGTGCACGCGGGGCTGGGGGATGCGTGTTATGTGGGGTTGTGCAG |
| Internal bar code: | TTGGATAACTATCATGGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTAAAGGGGGAGTTGGTG |
| Suggested primer 2: | GAGTGTGCCCTGTGCTTTTG |