| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060726 |
| Chromosome: | chromosome 1 |
| Location: | 6984249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049826 | PHC59 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCGGGGTGGATCCATGGACGGATGTACATTGGCAGCAAGAATGTTG |
| Internal bar code: | ATCGGCGTTTACGTAAGGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGCTCACCACCTTGTA |
| Suggested primer 2: | AAATGCTTGACACCAACCGC |