Insertion junction: CLIP2.060728_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060728
Chromosome:chromosome 3
Location:7551343
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre03.g205200 CSB21,TNP12 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCCGCTTCTGGCGCCGCCGCCGCGCTGTCCTGGCTCGCGGCTGCCAG
Internal bar code:TTGCAGCGGCCGGTATGGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1079
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCTCCTCTCTTCACGGC
Suggested primer 2:TCGTTGCAGACTTTGACCGA