| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060733 |
| Chromosome: | chromosome 12 |
| Location: | 1365422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489000 | (1 of 10) PF00170 - bZIP transcription factor (bZIP_1) | outside_mRNA | |
| Cre12.g489050 | PCT2,CDS2 | Phosphatidate cytidylyltransferase; (1 of 1) PTHR13773:SF3 - PHOSPHATIDATE CYTIDYLYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCCGAGCGGGTAGAGCGGATGCCGGTGAGATGAGCTGCTGTTTGACG |
| Internal bar code: | GATCGAATGGCTTAGGCTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5613 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTTGCTACGTCCACAC |
| Suggested primer 2: | GCCCTTCCTCAATGTGCTCT |