Insertion junction: CLIP2.060735_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060735
Chromosome:chromosome 16
Location:2409569
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g659700 3'UTR
Cre16.g659750 CEP15 Cysteine endopeptidase; (1 of 3) 3.4.18.1 - Cathepsin X / Lysosomal carboxypeptidase B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCTACCCCAATACATGCAAACGCAGTCGCTAATGCCACTTCACGCAC
Internal bar code:AAAATGGAAATCTAGTCGATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTGAGTTTTGGGGCGTG
Suggested primer 2:GTGGAGGCCGATGGAAAGAA