| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060744 |
| Chromosome: | chromosome 12 |
| Location: | 9882363 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543052 | (1 of 5) 1.14.13.9 - Kynurenine 3-monooxygenase / Kynurenine 3-hydroxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCTCCTGCACAGCCACACACTTTTGGTGCTGCCTCTTTGCCAGCTCC |
| Internal bar code: | AGTCAACGTCCATCCGCACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1974 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAGCCAGCGACTTGAGGG |
| Suggested primer 2: | TCTGGGGTACGTGGAGTAGG |