| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.060755 |
| Chromosome: | chromosome 3 |
| Location: | 833338 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146367 | (1 of 3) PTHR11697:SF74 - WITHOUT CHILDREN, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGTATACAACCCTGCGGAACATGTGGCTTTTGCCGTCTATCCTGCT |
| Internal bar code: | TCACATGGGCGTCGGCCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3810 |
| LEAP-Seq percent confirming: | 31.4815 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACAGCTGTTCGCAAGC |
| Suggested primer 2: | ACTCGGACTTCAACGGGAAC |