| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060756 |
| Chromosome: | chromosome 1 |
| Location: | 4865527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033900 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGAGGCGAAAAACCGGTGACGCGCCCGCTCTGCCGTGGAGGGATGC |
| Internal bar code: | CGTCGGTAAGGTGTTCGCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1820 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGGTGTGTGCGTACA |
| Suggested primer 2: | CTATGCAGTCCTACCAGCCG |