| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060762 |
| Chromosome: | chromosome 2 |
| Location: | 8144561 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144002 | PUS20 | (1 of 2) 5.4.99.24 - 23S rRNA pseudouridine(955/2504/2580) synthase; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTGTCCAGGACTGCGAAGGAGGTGGACGCCTCCTTGCCTTTGGGGC |
| Internal bar code: | ATGCTTTGGGGTATATTTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTCCCACCACCTTCAC |
| Suggested primer 2: | GCAGCCATCTATCACGACGA |