Insertion junction: CLIP2.060769_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060769
Chromosome:chromosome 12
Location:7069032
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g560150 RAB1,FAP353,RABD1 (1 of 1) K07874 - Ras-related protein Rab-1A (RAB1A); Rab-like GTP-Binding Flagellar Associated Protein 353 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTATTAATAGTCAATAGAGGAATAGTTGCGTGCTCCTGCTCTCTCTTCG
Internal bar code:GGAAAGCCGTCGATTGACGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4342
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:112

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGGATACACCAGAACACA
Suggested primer 2:TAGACGCTCTGAGGACTGCT