Insertion junction: CLIP2.060774_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060774
Chromosome:chromosome 8
Location:1768833
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g366600 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACGCTTTCCTTCATGCTTCCTCTTCTCAACTTCCCCCATTACTGGTT
Internal bar code:GAACATTACACCTAGTTCCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3194
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGCAACTAGAGGTCCAAT
Suggested primer 2:ACTGTACCGACGAGTTGCTG