| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060776 |
| Chromosome: | chromosome 14 |
| Location: | 3567775 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630859 | HID1 | 3-hydroxyisobutyrate dehydrogenase; (1 of 1) 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCAAAGAAGCGTGGTGGAGGCGGTGGTGGTGGGGTGGGTAGTTCGT |
| Internal bar code: | AAACGAACTCATAGTTAAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGTACCCAGCCATCCT |
| Suggested primer 2: | TGGACCCCGGATTAAAGTGC |