| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060782 |
| Chromosome: | chromosome 6 |
| Location: | 6610239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294600 | (1 of 3) PF15227 - zinc finger of C3HC4-type, RING (zf-C3HC4_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGTAGCCCCAGCTCGTCATCATGTCCTTTTACGGCGTTCCTGCTACA |
| Internal bar code: | CAGCGGCGGCTGATATAATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1618 |
| LEAP-Seq percent confirming: | 87.8505 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGATAGCGAGCTTAGCAA |
| Suggested primer 2: | TTGCTTGAGGACAGAGCCTG |