Insertion junction: CLIP2.060791_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060791
Chromosome:chromosome 16
Location:2913607
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663350 CLPX1,CLPX ClpX chaperone; (1 of 1) K03544 - ATP-dependent Clp protease ATP-binding subunit ClpX (clpX, CLPX) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCGTCACGGCAGCCTCAGATACAGTGGTAACGCTGCCGATACAGGGGC
Internal bar code:TTCGCTCATCGTCTAATTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4869
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:133

Suggested primers for confirmation by PCR

Suggested primer 1:GCTACCGCATTCGCTGAAAG
Suggested primer 2:CCTCCATTGAAGACGGCTGT