Insertion junction: CLIP2.060804_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.060804
Chromosome:chromosome 16
Location:5954958
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g678900 HIK,HKR6,HIK6 (1 of 3) IPR001789//IPR011006 - Signal transduction response regulator, receiver domain // CheY-like superfamily; Response regulator of two-component system 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCACACACATAGCACCTCCCTTAACCGCAACGCTGCCGCCCGTGCCA
Internal bar code:CATTGATGGCTGGGACCGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:6469
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:88
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACAGAAGGCTGCTGATC
Suggested primer 2:CATCAGACGAGATCAGGGCC