Insertion junction: CLIP2.060863_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060863
Chromosome:chromosome 16
Location:3307573
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g666500 BBS8 (1 of 1) K16781 - tetratricopeptide repeat protein 8 (TTC8, BBS8); Bardet Biedl Syndrome-8 associated protein 3'UTR
Cre16.g666550 SOUL3 SOUL heme-binding protein; (1 of 2) PTHR11220:SF35 - SOUL HEME-BINDING PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGAAGGCTGCCGACCTTCGCCAAACCGTGCATTGTTTCTGCGCACATA
Internal bar code:ACGGTTGGCAGCCCTTCCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2051
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TATTGGACGATGAGACGCCG
Suggested primer 2:AGCGCACGTAACAAGACTGA