| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060865 |
| Chromosome: | chromosome 10 |
| Location: | 138595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418600 | RLS3 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCCCCTCAGGACCAGCCGGCGTCAGCTTCACTGCCTTCCAGCGTTG |
| Internal bar code: | TGAACGTAGTAGAAGGGAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1708 |
| LEAP-Seq percent confirming: | 94.3396 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGGATGGCCCTAATACC |
| Suggested primer 2: | CAAAAGGATCATCTGCCGCG |