| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060901 |
| Chromosome: | chromosome 1 |
| Location: | 3180229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020223 | FUM2 | (1 of 1) K01679 - fumarate hydratase, class II (E4.2.1.2B, fumC); Fumarate hydratase 2 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCACGCTTCACGCTTGGTACCCTCCACGTACGTCCCATCCAGCCTCC |
| Internal bar code: | GCAGGATCGCTTAATTTCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1677 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCAAACACGCTCGCTTG |
| Suggested primer 2: | TCTTGCACTTGAAACGCACG |