| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060902 |
| Chromosome: | chromosome 12 |
| Location: | 4476354 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520400 | uL4m,MRPL4 | Mitochondrial ribosomal protein L4; (1 of 2) K02926 - large subunit ribosomal protein L4 (RP-L4, MRPL4, rplD) | 3'UTR |
| Cre12.g520450 | SGT1 | Serine O-galactosyltransferase 1; (1 of 1) PTHR31485:SF7 - PEPTIDYL SERINE ALPHA-GALACTOSYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACACCAACAGCCATTCTGCTCAATAGCAGCGCTTGCAGCGCGTTTCT |
| Internal bar code: | CCCGGGGATGCGCCTCATTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5180 |
| LEAP-Seq percent confirming: | 99.2857 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGAACATCCGCGTCGT |
| Suggested primer 2: | GCTGCCGCTATCTCTTGCTA |