Insertion junction: CLIP2.060904_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060904
Chromosome:chromosome 6
Location:3917213
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278192 (1 of 1) PTHR15627:SF8 - DTW DOMAIN-CONTAINING PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGTGGCTTTGGCTGCTGCAACCGACACACGGAGGGGCCGAGGGGGAGA
Internal bar code:ATGTAATAAATGTAGGCTGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:5831
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTATTGCGCTGTGAATG
Suggested primer 2:GGACGAGATTGCATTGCGAC