Insertion junction: CLIP2.060909_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060909
Chromosome:chromosome 8
Location:262054
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358563 (1 of 1) PTHR18934:SF85 - FI05376P 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAACGATTGAAGTCGAGGCAGACAGGCAGACTGCCGGCTGGTTACACA
Internal bar code:TGTGTTTCGAAAGTACAGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:438
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAAGTAGGCATCGCGAGT
Suggested primer 2:AGCACACGATTGGAGGAAGG