Insertion junction: CLIP2.060920_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.060920
Chromosome:chromosome 10
Location:6385350
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g464264 (1 of 1) PTHR13355:SF12 - GCN5-RELATED N-ACETYLTRANSFERASE (GNAT) FAMILY PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGCTCCCTGGGGGGCTTCTGGGAGCGCTGCTCCTTCGAAGACGACCG
Internal bar code:GACAGGTGAACCTGATTGTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:315
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACCAAGGCGCTTATGCATG
Suggested primer 2:CCAAATCCCGCCCACTAAGT