Insertion junction: CLIP2.060929_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060929
Chromosome:chromosome 5
Location:1487615
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234653 (1 of 781) IPR000104 - Antifreeze protein, type I 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAAATGGTTTACCATGCAGCCCACCCCTACCAATCCTGACCGGTCCT
Internal bar code:TACATCCTGTGCCTTTCCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3617
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGATTACGAGGAGGCC
Suggested primer 2:GCAATGTGTTGAGTGTGCGT