| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060940 |
| Chromosome: | chromosome 16 |
| Location: | 3013664 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663950 | ERG3 | Delta-7-sterol-C5(6)-desaturase; (1 of 1) K00227 - lathosterol oxidase (SC5DL, ERG3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACTGGGCAATTTCAGCATGGGTCCCCATTCCGACTCTGCAAACTGT |
| Internal bar code: | TCATTTTGGCGCAACTAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1322 |
| LEAP-Seq percent confirming: | 57.377 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGGGAGCATTGTCGTCT |
| Suggested primer 2: | CGTCCTATTGAGCGGTACCC |