Insertion junction: CLIP2.060946_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060946
Chromosome:chromosome 3
Location:6037797
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189950 HOP1,HOP HSP70-HSP90 organizing protein; (1 of 1) K09553 - stress-induced-phosphoprotein 1 (STIP1) 3'UTR
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTCTTCAGCTTCCAGTCCGCGCTATGGAAGTTTGGGGCGGGGATGCCC
Internal bar code:AAACTATTCAAGCAAGTCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3312
LEAP-Seq percent confirming:96.8254
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGGTCTGTTCTGCCGTA
Suggested primer 2:CTCTTGACAGAGGCGTTGGT