| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060951 |
| Chromosome: | chromosome 12 |
| Location: | 3429650 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497650 | THY28 | Predicted protein; (1 of 1) PF01878 - EVE domain (EVE) | 3'UTR |
| Cre12.g497652 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGGATTAAAATTCGGTAAGGGGCCATGGCTGATAAATGAGTGATTCC |
| Internal bar code: | GGAAGCTTACGCGAGTGCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2355 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACTACGACGAGCGCAGG |
| Suggested primer 2: | ATTACACCCTCCCTCCCCTC |