Insertion junction: CLIP2.060959_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.060959
Chromosome:chromosome 16
Location:3661826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677382 (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCATCTCGCCTGTACGGTTCCATTCTCTCACACCGTTGCGCCGCTCC
Internal bar code:GCACGGAGGAATGACCTACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2451
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGTAGCGGAAGTAGCGC
Suggested primer 2:GGCTGGCTCAGAAAGTTTGC