| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.060966 |
| Chromosome: | chromosome 6 |
| Location: | 2015145 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265300 | CSS2 | Chlamydomonas specific family S protein; (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGGTTTGTGCAGGGGCGATCTTACAGCAGTGGGCACGTGGGCTTGCG |
| Internal bar code: | TGTTAAATAGACGTAAGATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5598 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCCAACACAATGGCTGG |
| Suggested primer 2: | ATGACCCTGTCCATCTCCGA |